Table of Contents
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 1
How To Use This Catalog 2
Service Information 3-4
Placing Orders - Shipping 3
Miscellaneous Information 4
Product Information
Detergents 5-11
SOL-GRADE
®
Detergents 5
Kits 6-11
Custom Ampuling 11
Lipid-like Detergents 12-13
FOS-CHOLINE
®
Detergents 12
FOS-MEA
®
12
Miscellaneous 13
Nonionic Detergents 14-18
Sugar-based Detergents 14-15
Detergents Designed for Membrane Proteins 16
Polyoxyethylene Detergents 17-18
Sherpas
®
- Polymeric Solubilization Aids 19
Biochemicals 20-22
MTS Reagents 20
NDSB Reagents 21

Miscellaneous Biochemicals 22
Books 23
New Products 24
Alphabetical Product Listing 25-105
Technical Data
Detergent Analysis 106-107
Detergent Solubility 108
Detergent Grades 109
Detergent Stability 110
Detergent Properties Tables 111-116
Indexes
Catalog Number Index 117-118
Chemical Name Index 119-123
Registry Number Index 124-125
Catalog 2004/2005
1st Edition
February, 2004
*The image on the cover is a model of an octyl-β-D-glucoside micelle containing fifty mononers.
Please note that these micelles are nonspherical and nonuniform in shape and not all the hydro-
phobic tails are able to be buried in the center of the micelle. For more information, please read
the review article by Garavito and Ferguson-Miller plus the original work by Bogusz, Venable
and Pastor.
1. Bogusz, S., Venable, R. M. and Pastor, R. W., J. Phys. Chem. B 104, 5462-5470 (2000).
2. Garavito, R. M. and Ferguson-Miller, S., The Journal of Biological Chemistry 276
No. 35, 32403-32406 (2001).
2 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
How To Use This Catalog
Shipping and ordering information is given in the Service Information section on pages 3
and 4. Please note that we have changed our policy on shipping most detergents. Stability
studies indicate that a cold pack is not necessary with most of the reagents we offer,

including our high quality detergents (See page 110). For your convenience, we continue
to ship most of our products by overnight service for our
domestic customers and by air
for our international customers.
The Product Information section on pages 5-22 gives data on both new and old prod-
ucts. Look in this section for general information on detergent and other product areas.
The New Product section on page 24 lists all the products that have been added since
February 2003.
To locate a particular detergent or other reagent, please check the Alphabetical Product
Listing on pages 25-105 or use one of the three indexes available (Catalog Number
Index, pages 117-118; Chemical Name Index, pages 119-123 or Registry Number
Index, pages 124-125). Note that the Chemical Name Index lists a number of
common names for many of our products. A search function is also available at
www.anatrace.com.
Analytical procedures to determine purity of our detergents (Detergent Analysis) are
given on pages 106-107 and many detergent properties are listed in the Detergent
Properties Tables on pages 111-116.
It is our intention to upgrade our catalog every 3 to 6 months. If the date given on page
one is more than 6 months old, you may wish to download a new catalog at
www.anatrace.com, request one while at our web site or by calling our 800 number.
Service Information
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 3
Placing Orders
Telephone: Monday-Friday 8:00 AM - 5:00 PM (EST)
Toll free in the U.S. 800-252-1280
Or call 419-891-3030
Mail: Anatrace, Inc.
434 West Dussel Drive
Maumee, OH 43537
Fax: 419-891-3037

Internet: www.anatrace.com
Terms No minimum is required when ordering and all orders will be
shipped promptly, usually on the same day. Net 30 days.
Bulk Orders Should you need more than the largest size offered in the catalog,
there can be considerable savings by ordering in bulk. When
ordering please request special pricing.
Prices Although we make every effort to keep prices constant, the prices
herein may change without notice.
Credit Cards We accept VISA
®
and MasterCard
®
Shipping
Domestic Orders—Free Shipping on orders over $500.00.
All orders will be shipped by overnight service. When required, items will be shipped
overnight either with a cold pack or dry ice. Orders received before 1:00 PM EST will
generally be shipped the same day. A shipping and handling fee of $15.00 will be added
to the invoice of any order totaling less than $500.00.
International Orders—Free Shipping on orders over $500.00.
All orders will be shipped by express air. A shipping and handling fee of $35.00 will be
added to the invoice of any order totaling less than $500.00. Our international customers
are responsible for all duties and taxes at their location.
Please Note: Most detergents and other reagents offered by Anatrace have been shown
to be stable under normal shipping conditions and will NOT be shipped with either an ice
pack or dry ice. See Detergent Stability on page 110.
Service Information
4 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
Product Use
Anatrace products are supplied for laboratory use only. They are to be used for laboratory research and
experiments only. They may not be used for drugs, cosmetics, pesticide products or any use other than

stated above.
Quality
It is our goal to always supply the finest quality products available anywhere. If you are not completely
satisfied that the chemical supplied to you meets the specifications described herein, we will replace it.
Package Weight
Unless otherwise specified, the package will contain at least the indicated amount and usually slightly
more. The user is cautioned to always measure the required amount from the container.
Custom Synthesis
Our research staff will be pleased to work with you on the synthesis and purification of a novel detergent
or other reagent for your individual research needs.
Custom Ampuling and Reagent Preparation
Do you have a reagent that you must prepare frequently? Isn't it a waste of valuable lab time doing such
routine work? We at Anatrace are well accustomed to preparing reagents whether or not they contain
detergents. So consider giving Anatrace the opportunity to prepare your reagents.
- Volumes from 0.1 mL to 10 mL per ampul
- Each container flushed with inert gases, as requested
- Maximum container height of 85 mm
- Flame sealed
Orders as small as 100
Silk screen, transparent plastic, or opaque labels
Experienced with a wide range of reagents
Custom blending and preparation of reagents - or you supply the reagent
GMP procedures followed throughout
Technical Support
For detailed information and/or technical assistance call our toll free number during normal business hours:
800-252-1280. Inquiries can also be sent by FAX: 419-891-3037 or e-mail:
Return Policy
Should you receive a product ordered from this catalog which is believed to be nonconforming, please
return the material within 30 days. You must receive authorization from Anatrace before returning the
product. Returned products that have been ordered in error or have been retained more than 30 days

are subject to a 25% restocking fee.
Product Information
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 5
Anatrace Offers Solubilization Grade Detergents
SOL-GRADE
In response to customer requests, we are introducing a grade of detergents designed specifically
for the extraction of membrane proteins—still chromatographically purified to greater than 97%
or 98% and at a savings of about 20–25% over the cost of ANAGRADE
®
products—probably
even greater savings if you are currently using a competitor's product. See the alphabetical
product listing for more information on a specific product.
C316S CHAPS, SOL-GRADE
®
35
C325S CYMAL
®
-5, SOL-GRADE
®
45
D322S n-DECYL
−−
−−
−
ββ
ββ
β
−−
−−

−D-MALTOPYRANOSIDE, SOL-GRADE
®
50
D350S n-DODECYL-N,N-DIMETHYLGLYCINE, SOL-GRADE
®
55
D360S n-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE, SOL-GRADE
®
55
D310S n-DODECYL
−−
−−
−
ββ
ββ
β
−−
−−
−D-MALTOPYRANOSIDE, SOL-GRADE
®
59
F304S FOS-CHOLINE
®
-10, SOL-GRADE
®
62
F306S FOS-CHOLINE
®
-11, SOL-GRADE
®

63
F308S FOS-CHOLINE
®
-12, SOL-GRADE
®
64
F312S FOS-CHOLINE
®
-14, SOL-GRADE
®
65
F316S FOS-CHOLINE
®
-16, SOL-GRADE
®
66
M310S 6-O-METHYL-n-HEPTYLCARBOXYL-
αα
αα
α-D-GLUCOPYRANOSIDE, SOL-GRADE
®
77
N324S n-NONYL
−−
−−
−
ββ
ββ
β
−−

−−
−D-GLUCOPYRANOSIDE, SOL-GRADE
®
79
O311S n-OCTYL
−−
−−
−
ββ
ββ
β
−−
−−
−D-GLUCOPYRANOSIDE, SOL-GRADE
®
84
O310S n-OCTYL
−β−−β−
−β−−β−
−β−D-MALTOPYRANOSIDE, SOL-GRADE
®
86
S1010S SODIUM CHOLATE, SOL-GRADE
®
92
S300S SODIUM DODECANOYL SARCOSINE, SOL-GRADE
®
93
T315S n-TETRADECYL
−β−−β−

−β−−β−
−β−D-MALTOPYRANOSIDE, SOL-GRADE
®
96
T323S n-TRIDECYL
−β−−β−
−β−−β−
−β−D-MALTOPYRANOSIDE, SOL-GRADE
®
99
U300S n-UNDECYL
−−
−−
−
ββ
ββ
β
−−
−−
−D-MALTOPYRANOSIDE, SOL-GRADE
®
102
Product Information
INDEX PRODUCT LISTING NEW PRODUCTS PRODUCT INFOMATION
6 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
Kits
To select the optimum detergent or combination of detergents and reagents for a particular
membrane protein usually requires testing of many detergents. Note also that the detergent
which is suitable for extraction may not be useful for storage of the purified protein or for
studies conducted on the purified protein. To aid in the selection process, Anatrace offers

a wide variety of solid and solution based detergent kits.
Listed below are nonionic and ionic Master Detergent Kits which contain one gram of each
detergent. Following the master kits are several smaller kits which likewise contain one
gram of the specified detergents.
Please Note: Kit pricing represents a substantial discount.
D399-ND69 NONIONIC MASTER DETERGENT KIT 2784.62
Contains one gram of each of the detergents listed below:
ANAMEG
®
-7
Big CHAP
Big CHAP, Deoxy
C-HEGA
®
-8
C-HEGA
®
-9
C-HEGA
®
-10
C-HEGA
®
-11
CYGENT
™
1-3
CYGLU
®
-3

CYGLU
®
-4
CYMAL
®
-1
CYMAL
®
-2
CYMAL
®
-3
CYMAL
®
-4
CYMAL
®
-5
CYMAL
®
-6
CYMAL
®
-7
2,6-Dimethyl-4-heptyl-β-
D-maltopyranoside
6-O-Methyl-n-heptylcarboxyl-
α-D-glucopyranoside
HEGA
®

-8
HEGA
®
-9
HEGA
®
-10
HEGA
®
-11
n-Hexyl-β-D-glucopyranoside
n-Heptyl-β-D-glucopyranoside
n-Octyl-α-D-glucopyranoside
n-Octyl-β-D-glucopyranoside
n-Nonyl-β-D-glucopyranoside
n-Decyl-β-D-glucopyranoside
n-Dodecyl-α-D-glucopyranoside
n-Dodecyl-β-D-glucopyranoside
n-Hexyl-β-D-maltopyranoside
n-Octyl-β-D-maltopyranoside
n-Nonyl-β-D-maltopyranoside
n-Decyl-α-D-maltopyranoside
n-Decyl-β-D-maltopyranoside
n-Undecyl-α-D-maltopyranoside
n-Undecyl-β-D-maltopyranoside
n-Dodecyl-α-D-maltopyranoside
n-Dodecyl-β-D-maltopyranoside
n-Tridecyl-β-D-maltopyranoside
n-Tetradecyl-β-D-maltopyranoside
n-Hexadecyl-β-D-maltopyranoside

ω-Undecylenyl-β-D-maltopyranoside
n-Heptyl-β-D-thioglucopyranoside
n-Octyl-β-D-thioglucopyranoside
n-Nonyl-β-D-thioglucopyranoside
n-Decyl-β-D-thioglucopyranoside
n-Octyl-β-D-thiomaltopyranoside
n-Nonyl-β-D-thiomaltopyranoside
n-Decyl-β-D-thiomaltopyranoside
n-Undecyl-β-D-thiomaltopyranoside
n-Dodecyl-β-D-thiomaltopyranoside
MEGA-8
MEGA-9
MEGA-10
Poloxamer
®
407
2-Propyl-1-pentyl-β-D-
maltopyranoside
Hexaethylene glycol monooctyl
ether (C
8
E
6
) (50% W/W)
Octaethylene glycol monododecyl
ether (C
12
E
8
) (25% W/W)

Pentaethylene glycol monodecyl
ether (C
10
E
5
) (50% W/W)
Pentaethylene glycol monooctyl
ether (C
8
E
5
) (50% W/W)
Tetraethylene glycol monooctyl
ether (C
8
E
4
) (50% W/W)
n-Octyl-β-D-galactopyranoside
Sucrose monododecanoate
Dimethyldecylphosphine oxide
Dimethyloctylphosphine oxide
n-Tetradecyl-N,N-dimethylamine-
N-oxide (TDAO)
n-Dodecyl-N,N-dimethylamine-
N-oxide (DDAO)
Product Information
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 7
D399-CE ALKYL POLYOXYETHYLENE (C

X
E
Y
) DETERGENT KIT 152.33
Kit contains Hexaethylene glycol monooctyl ether (C
8
E
6
) (50% W/W solution),
Octaethylene glycol monododecyl ether (C
12
E
8
) (25% W/W solution), Pentaethylene
glycol monodecyl ether (C
10
E
5
) (50% W/W solution) and Tetraethylene glycol
monooctyl ether (C
8
E
4
) (50% W/W solution) ampuled and stored under Argon.
D399-ANZ ANZERGENT
®

DETERGENT KIT 39.00
Kit contains ANZERGENT
®

3-8, 3-10, 3-12 and 3-14.
D399-CY25 CYCLOFOS
™
DETERGENT KIT (2-5) 335.87
Kit contains CYCLOFOS
™
-2, 3, 4 and 5.
D399-C14 CYMAL
®
DETERGENT KIT (1-4) 190.30
Kit contains CYMAL
®
-1, 2, 3 and 4.
D399-F812 FOS-CHOLINE
®
DETERGENT KIT (8-12) 365.34
Kit contains FOS-CHOLINE
®
8, 9, 10, 11 and 12.
D399-FM812 FOS-MEA
®
DETERGENT KIT 234.54
Kit contains FOS-MEA
®
-8, 10 and 12.
D399-G GLUCOPYRANOSIDE DETERGENT KIT 187.21
Kit contains n-Hexyl-β-D-glucopyranoside, n-Heptyl-β-D-glucopyranoside,
n-Octyl-β-D-glucopyranoside, n-Nonyl-β-D-glucopyranoside, n-Decyl-β-D-
glucopyranoside and n-Dodecyl-β-D-glucopyranoside.
D399-ID59 IONIC MASTER DETERGENT KIT 3162.22

Contains one gram of each of the detergents listed below:
ANZERGENT
®
3-8
ANZERGENT
®
3-10
ANZERGENT
®
3-12
ANZERGENT
®
3-14
C-DODECAFOS
™
C-OCTAFOS
™
3-Carboxy-ω-heptadecen-
amidopropyl dimethylamine
3-Carboxy-5-pentadecen-
amidopropyl dimethylamine
CHAPS
CHAPSO
CYCLOFOS
™
-2
CYCLOFOS
™
-3
CYCLOFOS

™
-4
CYCLOFOS
™
-5
CYCLOFOS
™
-6
CYCLOFOS
™
-7
CYPFOS
™
-3
FOS-CHOLINE
®
-8
FOS-CHOLINE
®
-9
FOS-CHOLINE
®
-10
FOS-CHOLINE
®
-11
FOS-CHOLINE
®
-12
FOS-CHOLINE

®
-13
FOS-CHOLINE
®
-14
FOS-CHOLINE
®
-15
FOS-CHOLINE
®
-16
FOS-CHOLINE
®
-ISO-9
FOS-CHOLINE
®
-ISO-11
FOS-CHOLINE
®
-ISO-11-6U
FOS-CHOLINE
®
-UNSAT-11-10
FOS-MEA
®
-8
FOS-MEA
®
-10
FOS-MEA

®
-12
FOSFEN
™
-9
FOSFENETH
™
-4
NOPOL-FOS
™
PMAL
™
-C4
PMAL
™
-C6
PMAL
™
-C8
PMAL
™
-C10
PMAL
™
-C12
PMAL
™
-C16
PMALA
™

-C12
Decyltrimethylammonium chloride
Dodecyltrimethylammonium chloride
Hexadecyltrimethylammonium chloride
Octadecyltrimethylammonium chloride
Tetradecyltrimethylammonium chloride
n-Decyl-N,N-dimethylglycine
n-Dodecyl-N,N-dimethylglycine
n-Tetradecyl-N,N-dimethylglycine
n-Dodecyl-β-iminodipropionic acid,
disodium salt
n-Dodecyl-β-iminodipropionic acid,
monosodium salt
Deoxycholic acid, sodium salt
Sodium cholate
Sodium decanoyl sarcosine
Sodium dodecanoyl sarcosine
Sodium dodecyl sulfate
Taurocholic Acid
Product Information
INDEX PRODUCT LISTING NEW PRODUCTS PRODUCT INFOMATION
8 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
D399-HA HIGH ALPHA DETERGENT KIT 276.86
Kit contains n-Decyl-α-D-maltopyranoside, n-Dodecyl-α-D-glucopyranoside,
n-Dodecyl-α-D-maltopyranoside, n-Octyl-α-D-glucopyranoside and
n-Undecyl-α-D-maltopyranoside.
D399-ION IONIC DETERGENT KIT 106.67
Kit contains Sodium cholate, n-Dodecyl-N,N-dimethylglycine, n-Decyl-N,N-
dimethylglycine, Sodium dodecanoyl sarcosine, Sodium decanoyl sarcosine and
Deoxycholic acid, Sodium salt.

D399-M611 MALTOPYRANOSIDE DETERGENT KIT (6-11) 174.80
Kit contains n-Hexyl-β-D-maltopyranoside, n-Octyl-β-D-maltopyranoside, n-Nonyl-
β-D-maltopyranoside, n-Decyl-β-D-maltopyranoside and n-Undecyl-β-D-
maltopyranoside.
D399-M1216 MALTOPYRANOSIDE DETERGENT KIT (12-16) 236.77
Kit contains n-Dodecyl-β-D-maltopyranoside, n-Tridecyl-β-D-maltopyranoside,
n-Tetradecyl-β-D-maltopyranoside and n-Hexadecyl-β-D-maltopyranoside.
D399-MG MEGA DETERGENT KIT 56.09
Kit contains MEGA-8, 9 and 10.
D399-MSF MISCELLANEOUS FOS DETERGENT KIT 416.94
Kit contains FOSFENETH
™
-4, FOS-CHOLINE
®
-ISO-9, NOPOL-FOS
™
,
C-DODECAFOS
™
and CYPFOS
™
-3.
D399-MSFU MISCELLANEOUS FOS-CHOLINE
®
DETERGENT KIT 130.30
Kit contains FOS-CHOLINE
®
-ISO-11, FOS-CHOLINE
®
-ISO-11-6U and

FOS-CHOLINE
®
-UNSAT-11-10.
D399-OX OXIDE DETERGENT KIT 63.32
Kit contains Dimethyldecylphosphine oxide, n-Tetradecyl-N,N-dimethylamine-N-
oxide (TDAO) and n-Dodecyl-N,N-dimethylamine-N-oxide (DDAO).
D399-PM48 PMAL
™
DETERGENT KIT (4-8) 249.00
Kit contains PMAL
™
-C4, PMAL
™
-C6 and PMAL
™
-C8.
D399-PM1016 PMAL
™
DETERGENT KIT (10-16) 249.00
Kit contains PMAL
™
-C10, PMAL
™
-C12 and PMAL
™
-C16.
D399-POLY POPULAR POLYMER DETERGENT KIT 269.00
Kit contains PMAL
™
-C10, PMAL

™
-C12, PMALA
™
-C12 and Poloxamer
®
407.
D399-POP POPULAR DETERGENT KIT 211.99
Kit contains CHAPS, CYMAL
®
-5, FOS-CHOLINE
®
-12, n-Decyl-β-D-maltopyranoside,
n-Dodecyl-β-D-maltopyranoside and n-Octyl-β-D-glucopyranoside.
D399-QUAT QUATERNARY DETERGENT KIT 29.00
Kit contains Decyltrimethylammonium chloride, Dodecyltrimethylammonium chloride,
Hexadecyltrimethylammonium chloride, Octadecyltrimethylammonium chloride, and
Tetradecyltrimethylammonium chloride.
D399-TG THIOGLUCOPYRANOSIDE DETERGENT KIT 144.19
Kit contains n-Heptyl-β-D-thioglucopyranoside, n-Octyl-β-D-thioglucopyranoside,
n-Nonyl-β-D-thioglucopyranoside and n-Decyl-β-D-thioglucopyranoside.
D399-TM THIOMALTOPYRANOSIDE DETERGENT KIT 236.91
Kit contains n-Octyl-β-D-thiomaltopyranoside, n-Nonyl-β-D-thiomaltopyranoside,
n-Decyl-β-D-thiomaltopyranoside, n-Dodecyl-β-D-thiomaltopyranoside and
n-Undecyl-β-D-Thiomaltopyranoside.
Product Information
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 9
Soluble Detergent Kits
Save Time and Money
Anatrace detergents are now available in kits as ampuled 10% solutions stored under argon. These

solution kits cost a fraction of the one gram detergent kits, making the selection of the “right” deter-
gent for extraction of a membrane protein less expensive. The master kit listed below contains nearly
every detergent offered by Anatrace that possesses sufficient solubility to prepare a 10% solution.
DSOL-114-10 SOLUTION MASTER DETERGENT KIT 979.00
Contains one ampule (one ml of a 10% solution) of each detergent listed below:
ANAMEG
®
-7
ANAPOE
®
-20
ANAPOE
®
-35
ANAPOE
®
-58
ANAPOE
®
-80
ANAPOE
®
-C
10
E
6
ANAPOE
®
-C
10

E
9
ANAPOE
®
-C
12
E
8
ANAPOE
®
-C
12
E
9
ANAPOE
®
-C
12
E
10
ANAPOE
®
-C
13
E
8
ANAPOE
®
-X-100
ANAPOE

®
-X-114
ANAPOE
®
-X-305
ANAPOE
®
-X-405
ANZERGENT
®
3-8
ANZERGENT
®
3-10
ANZERGENT
®
3-12
ANZERGENT
®
3-14
Big CHAP
Big CHAP, deoxy
CHAPS
CHAPSO
Deoxycholic acid, sodium salt
Sodium cholate
C-DODECAFOS
™
C-HEGA
®

-8
C-HEGA
®
-9
C-HEGA
®
-10
C-HEGA
®
-11
CYCLOFOS
™
-2
CYCLOFOS
™
-3
CYCLOFOS
™
-4
CYCLOFOS
™
-5
CYCLOFOS
™
-6
CYCLOFOS
™
-7
CYGLU
®

-3
CYMAL
®
-1
CYMAL
®
-2
CYMAL
®
-3
CYMAL
®
-4
CYMAL
®
-5
CYMAL
®
-6
CYMAL
®
-7
CYPFOS
™
-3
2,6-Dimethyl-4-heptyl-β-D-
maltopyranoside
2-Propyl-1-pentyl
maltopyranoside
FOS-CHOLINE

®
-8
FOS-CHOLINE
®
-9
FOS-CHOLINE
®
-10
FOS-CHOLINE
®
-11
FOS-CHOLINE
®
-12
FOS-CHOLINE
®
-13
FOS-CHOLINE
®
-14
FOS-CHOLINE
®
-15
FOS-CHOLINE
®
-16
FOS-CHOLINE
®
-ISO-9
FOS-CHOLINE

®
-ISO-11
FOS-CHOLINE
®
-ISO-11-6U
FOS-CHOLINE
®
-UNSAT-11-10
FOS-MEA
®
-8
FOS-MEA
®
-10
FOSFEN
™
-9
FOSFENETH
™
-4
HEGA
®
-8
HEGA
®
-9
HEGA
®
-10
MEGA-8

NOPOL-FOS
™
PMAL
™
-C4
PMAL
™
-C6
PMAL
™
-C8
PMAL
™
-C10
PMAL
™
-C12
Decyltrimethylammonium chloride
Dodecyltrimethylammonium chloride
Hexadecyltrimethylammonium chloride
Octadecyltrimethylammonium chloride
Tetradecyltrimethylammonium chloride
n-Hexyl-β-D-glucopyranoside
n-Heptyl-β-D-glucopyranoside
n-Octyl-β-D-glucopyranoside
n-Nonyl-β-D-glucopyranoside
n-Hexyl-β-D-maltopyranoside
n-Octyl-β-D-maltopyranoside
n-Nonyl-β-D-maltopyranoside
n-Decyl-α-D-maltopyranoside

n-Decyl-β-D-maltopyranoside
n-Undecyl-α-D-maltopyranoside
n-Undecyl-β-D-maltopyranoside
n-Dodecyl-α-D-maltopyranoside
n-Dodecyl-β-D-maltopyranoside
n-Tridecyl-β-D-maltopyranoside
n-Tetradecyl-β-D-maltopyranoside
n-Heptyl-β-D-thioglucopyranoside
n-Octyl-β-D-thiomaltopyranoside
n-Nonyl-β-D-thiomaltopyranoside
n-Decyl-β-D-thiomaltopyranoside
n-Undecyl-β-D-thiomaltopyranoside
n-Dodecyl-β-D-thiomaltopyranoside
n-Decyl-N,N-dimethylglycine
n-Dodecyl-N,N-dimethylglycine
n-Dodecyl-β-iminodipropionic acid,
disodium salt
n-Dodecyl-β-iminodipropionic acid,
monosodium salt
Sodium decanoyl sarcosine
Sodium dodecanoyl sarcosine
Hexaethylene glycol monooctyl ether (C
8
E
6
)
Octaethylene glycol monododecyl ether (C
12
E
8

)
Pentaethylene glycol monodecyl ether (C
10
E
5
)
Tetraethylene glycol monooctyl ether (C
8
E
4
)
Sucrose monododecanoate
Dimethyldecylphosphine oxide
n-Tetradecyl-N,N-dimethylamine-N-
oxide (TDAO)
n-Dodecyl-N,N-dimethylamine-N-
oxide (DDAO)
Product Information
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Additional kits containing detergents as 10% aqueous solutions are listed below:
DSOL-ANAP1 ANAPOE
®
MASTER KIT (1 mL size) 29.00
Kit contains 1 mL of all 14 ANAPOE
®
detergents
DSOL-ANP10 ANAPOE
®
MASTER KIT (10 mL size) 79.00

Kit contains 10 mL of all 14 ANAPOE
®
detergents
DSOL-ANPC ANAPOE
®
CYEX KIT (10 mL size) 49.00
Kit contains 10 mL of ANAPOE
®
-35, ANAPOE
®
-58, ANAPOE
®
-C
10
E
6
,
ANAPOE
®
-C
10
E
9
, ANAPOE
®
-C
12
E
8
, ANAPOE

®
-C
12
E
9
, ANAPOE
®
-C
12
E
10
,
and ANAPOE
®
-C
13
E
8
.
DSOL-ANPT ANAPOE
®
TRITON/TWEEN KIT (10 mL size) 29.00
Kit contains 10 mL of ANAPOE
®
-X-100, ANAPOE
®
-X-114, ANAPOE
®
-X-305,
ANAPOE

®
-X-405, ANAPOE
®
-20, and ANAPOE
®
-80.
DSOL-ANZ ANZERGENT
®
DETERGENT KIT 29.00
Kit contains ANZERGENT
®
3-8, 3-10, 3-12 and 3-14.
DSOL-BILE BILE ACID-BASED DETERGENT KIT 59.00
Kit contains CHAPS, CHAPSO, Sodium cholate, Big CHAP, Big CHAP, deoxy
and Deoxycholic acid, sodium salt.
DSOL-CY25 CYCLOFOS
™
DETERGENT KIT (2-5) 79.00
Kit contains CYCLOFOS
™
-2, 3, 4 and 5.
DSOL-C14 CYMAL
®
DETERGENT KIT (1-4) 59.00
Kit contains CYMAL
®
-1, 2, 3 and 4.
DSOL-C57 CYMAL
®
DETERGENT KIT (5-7) 69.00

Kit contains CYMAL
®
-5, 6 and 7.
DSOL-F812 FOS-CHOLINE
®
DETERGENT KIT (8-12) 69.00
Kit contains FOS-CHOLINE
®
-8, 9, 10, 11 and 12.
DSOL-F1316 FOS-CHOLINE
®
DETERGENT KIT (13-16) 69.00
Kit contains FOS-CHOLINE
®
-13, 14, 15 and 16.
DSOL-G GLUCOPYRANOSIDE DETERGENT KIT 59.00
Kit contains n-Hexyl−β−D-glucopyranoside, n-Heptyl−β−D-glucopyranoside,
n-Octyl−β−D-glucopyranoside and n-Nonyl−β−D-glucopyranoside.
DSOL-HA HIGH ALPHA DETERGENT KIT 69.00
Kit contains n-Decyl-α-D-maltopyranoside, n-Dodecyl-α-D-glucopyranoside,
n-Dodecyl-α-D-maltopyranoside, n-Octyl-α-D-glucopyranoside and
n-Undecyl-α-D-maltopyranoside .
DSOL-ION IONIC DETERGENT KIT 39.00
Kit contains Sodium cholate, n-Dodecyl-N,N-dimethylglycine, n-Decyl-N,N-
dimethylglyine,Sodium dodecanoyl sarcosine, Sodium decanoyl sarcosine and
Deoxycholic acid, sodium salt.
DSOL-M611 MALTOPYRANOSIDE DETERGENT KIT 59.00
Kit contains n-Hexyl−β−D-maltopyranoside, n-Octyl−β−D-maltopyranoside,
n-Nonyl−β−D-maltopyranoside, n-Decyl−β−D-maltopyranoside and
n-Undecyl−β−D-maltopyranoside.

DSOL-M1214 MALTOPYRANOSIDE DETERGENT KIT 59.00
Kit contains n-Dodecyl−β−D-maltopyranoside, n-Tridecyl−β−D-maltopyranoside
and n-Tetradecyl−β−D-maltopyranoside.
Product Information
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DSOL-MS MISCELLANEOUS DETERGENT KIT 69.00
Kit contains CHAPS, FOS-CHOLINE
®
-ISO-9, CHAPSO, DDAO,
CYPFOS
™
-3 and TDAO.
DSOL-MSS MISCELLANEOUS SUGAR-BASED DETERGENT KIT 79.00
Kit contains 2,6 Dimethyl-4-heptyl−β−D-maltopyranoside, ANAMEG
®
-7,
Sucrose monododecanoate, CYGLU
®
-3, Big CHAP, and Big CHAP, deoxy.
DSOL-OX OXIDE DETERGENT KIT 49.00
Kit contains Dimethyldecylphosphine oxide, n-Tetradecyl-N,N-dimethylamine-
N-oxide (TDAO) and n-Dodecyl-N,N-dimethylamine-N-oxide (DDAO).
DSOL-PM PMAL
™
DETERGENT KIT 99.00
Kit contains PMAL
™
-C4, PMAL
™

-C6, PMAL
™
-C8, PMAL
™
-C10
and PMAL
™
-C12.
DSOL-CE POLYOXYETHYLENE DETERGENT KIT 59.00
Kit contains C
8
E
4
, C
8
E
6
, C
10
E
5
and C
12
E
8
.
DSOL-POP POPULAR DETERGENT KIT 69.00
Kit contains CHAPS, CYMAL
®
-5, n-Decyl−β−D-maltopyranoside,

n-Dodecyl−β−D-maltopyranoside, FOS-CHOLINE
®
-12, and
n-Octyl−β−D-glucopyranoside.
DSOL-QUAT QUATERNARY DETERGENT KIT 29.00
Kit contains Decyltrimethylammonium chloride, Dodecyltrimethylammonium chloride,
Hexadecyltrimethylammonium chloride, Octadecyltrimethylammonium chloride, and
Tetradecyltrimethylammonium chloride.
DSOL-TM THIOMALTOPYRANOSIDE DETERGENT KIT 69.00
Kit contains n-Octyl−β−D-Thiomaltopyranoside, n-Nonyl−β−D-Thiomaltopyranoside,
n-Decyl−β−D-Thiomaltopyranoside, n-Dodecyl−β−D-Thiomaltopyranoside and
n-Undecyl−β−D-Thiomaltopyranoside.
Custom Ampuling and Reagent Preparation
Do you have a reagent that you must prepare frequently? Isn't it a waste of valuable lab time doing such routine
work? We at Anatrace are well accustomed to preparing reagents whether or not they contain detergents. So
consider giving Anatrace the opportunity to prepare your reagents.
- Volumes from 0.1 mL to 10 mL per ampul
- Each container flushed with inert gases, as requested
- Maximum container height of 85 mm
- Flame sealed
Orders as small as 100
Silk screen, transparent plastic, or opaque labels
Experienced with a wide range of reagents
Custom blending and preparation of reagents - or you supply the reagent
GMP procedures followed throughout
Product Information
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12 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
Lipid-like Detergents
Anatrace is pleased to offer more than twenty phospholipid analogs. Support for the development effort came from

NIDDK through a Small Business Innovative Research (SBIR) grant (NIDDK 1R43 DK49911-02).
FOS-CHOLINE
®
detergents
The detergents in this series have the same head groups as phospholipids, but unlike the phospholipids they possess
simple hydrophobic tails.
n = 7, FOS-CHOLINE
®
-8
n = 8, FOS-CHOLINE
®
-9
n = 9, FOS-CHOLINE
®
-10
n = 10, FOS-CHOLINE
®
-11
n = 11, FOS-CHOLINE
®
-12
n = 12, FOS-CHOLINE
®
-13
n = 13, FOS-CHOLINE
®
-14
n = 14, FOS-CHOLINE
®
-15

n = 15, FOS-CHOLINE
®
-16
The lack of a complex glycerol ester chain allows these detergents to be prepared and offered to the researcher at
moderate prices, compared to many phospholipids. Note also that there are no chiral centers in these detergents as
there are in the phospholipids and most phospholipid derivatives. FOS-CHOLINE
®
-12 (n-dodecylphosphocholine)
micelles have proven to be extremely useful as a medium in which to conduct NMR studies of membrane proteins
1,2
and also play a crucial role in a method recently forwarded for refolding a misfolded membrane protein.
3
FOS-MEA
®
detergents
The FOS-MEA
®
detergents have a secondary amine in the head group instead of the quaternary amine found in the
FOS-CHOLINE
®
detergents:
n = 7, FOS-MEA
®
-8
n = 9, FOS-MEA
®
-10
n = 11, FOS-MEA
®
-12

Because of the replacement of the quaternary amine with a secondary amine, this series has a lower solubility than the
FOS-CHOLINE
®
detergents.
CYCLOFOS
™
detergents
CYCLOFOS
™
detergents combine the phospholipid head group with an aliphatic tail containing a cyclohexyl group
as present in the CYMAL
®
series of detergents.
n = 2, CYCLOFOS
™
-2
n = 3, CYCLOFOS
™
-3
n = 4, CYCLOFOS
™
-4
n = 5, CYCLOFOS
™
-5
n = 6, CYCLOFOS
™
-6
n = 7, CYCLOFOS
™

-7
(CH
2
)n
+
(CH
2
)
2
N(CH
3
)
3
O
OPO
_
O
OO P
O
_
+
O
(CH
2
)
2
N(CH
3
)
3

CH
3
(CH
2
)
n
H
N
CH
3
(CH
2
)
2
O
O
OH
P
O
CH
3
(CH
2
)
n
Product Information
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FOSFEN
™

-9
FOSFEN
™
-9 is another lipid-like detergent containing a phenyl ring:
Note: FOSFEN
™
-9 is prepared from a technical grade mixture of monoalkylphenols, predominantly para substituted.
For more information see Merck Index 12,6775.
n = 8, FOSFEN
™
-9
Other lipid-like detergents
The chemists at Anatrace continue to explore unique structures which show promise for use in extraction and
crystallization of membrane proteins. Several detergents which illustrate variations of the hydrophobic portion of
the detergent are listed below.
FOS-CHOLINE
®
-ISO-9, FOS-CHOLINE
®
-ISO-11
To the phospholipid analog detergents discussed above, Anatrace has added FOS-CHOLINE
®
-ISO-9 and
FOS-CHOLINE
®
-ISO-11 which possess a split hydrophobic tail packing more hydrophobicity into a shorter
effective chain length. This detergent may be useful for the stabilization of proteins during crystallization.
n = 1, FOS-CHOLINE
®
-ISO-9

n = 2, FOS-CHOLINE
®
-ISO-11
FOS-CHOLINE
®
-UNSAT-11-10
FOS-CHOLINE
®
-UNSAT-11-10 contains an eleven carbon alkyl tail with a double bond at the
end of the chain:
1. K. Oxenoid, F. D. Soennichsen and C. R. Sanders, Biochemistry 40, 5111-5118, (2001).
2. K. Oxenoid and C. R. Sanders, Biochimica et Biophysica Acta 1508, 129-145, (2000).
3. B. M. Gorzelle, J. K. Nagy, K. Oxenoid, W. L. Lonzer, D. S. Cafiso and C. R. Sanders, Biochemistry 38, 16373-16382, (1999).
OP
O
O
O
N(CH
3
)
3
(CH
2
)
n
(CH
2
)
n
CH

3
CH
3
CH
3
CH
3
_
+
CH
CH
CH
(CH
2
)
2
P
O
O
O
(CH
2
)
2
N(CH
3
)
3
O
(CH

2
)
n
CH
3
_
+
CH
2
CH (CH
2
)
9
O
PO
(CH
2
)
2
N(CH
3
)
3
O
O
_
+
Product Information
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14 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com

Nonionic Detergents
Most of the detergents useful in the extraction and purification of membrane proteins are Nonionic detergents which
possess either a sugar based or a polyoxyethylene head group.
Sugar-based detergents
Sugar-based detergents are among the most popular type of detergents used with membrane proteins. The alkyl
glucopyranoside series is available from the six carbon chain tail to dodecyl-β-D-glucopyranoside. The somewhat
less polar thioglycoside analogs of some of these compounds are also available.
The high CMC value of hexyl-β-D-glucopyranoside (0.25M) indicates that a detergent with a shorter chain would
not be useful. Decyl-β-D-glucopyranoside has a reasonable CMC value, but is marginally soluble. Dodecyl-β-D-
glucopyranoside is even less soluble.
For many membrane proteins, a member of the maltopyranoside series is milder and more effective than the compa-
rable member of the glucopyranoside series.
n = 5, hexyl−β−D-glucopyranoside
n = 6, heptyl−β−D-glucopyranoside
n = 7, octyl−β−D-glucopyranoside
n = 8, nonyl−β−D-glucopyranoside
n = 9, decyl−β−D-glucopyranoside
n = 11, dodecyl−β−D-glucopyranoside
n = 5, hexyl−β−D-maltopyranoside
n = 7, octyl−β−D-maltopyranoside
n = 8, nonyl−β−D-maltopyranoside
n = 9, decyl−β−D-maltopyranoside
n = 10, undecyl−β−D-maltopyranoside
n = 11, dodecyl−β−D-maltopyranoside
n = 12, tridecyl−β−D-maltopyranoside
n = 13, tetradecyl−β−D-maltopyranoside
n = 15, hexadecyl−β−D-maltopyranoside
Another advantage of the maltopyranoside head group is the increased solubility of the higher members of this
series compared to the glucopyranoside series. Even hexadecyl-β-D-maltopyranoside is soluble although it must
be heated to 50

o
C for a few minutes to make a “true” solution. The entire series of maltopyranoside detergents
are available from hexyl-β-D-maltopyranoside to hexadecyl-β-D-maltopyranoside, except for the pentadecyl
member.
Since the maltopyranoside detergents are generally milder than the glucopyranoside detergents, one might think
that maltotrioside detergents might be even more compatible with membrane proteins. Detergents with even larger
head groups seem to be impractical because the higher polysaccharides are not available in pure form at a reason-
able price.
Two sugar-based detergents that have been available for some time are sucrose monododecanoate and sucrose
monodecanoate. As with the maltose-based detergent series, these detergents are mild and useful for the extrac-
tion of membrane proteins. The researcher should be aware, however, that these detergents are esters rather than
glycosidic compounds and thus are easily hydrolyzed in acid, base or by proteases. The sucrose ester detergents
are also mixtures of isomers in which the aliphatic ester is linked to any one of the three primary alcohols in the
sucrose structure.
O
OH
HO
OH
CH
2
OH
O
(CH
2
)
n
CH
3

O

OH
HO
OH
CH
2
OH
O
OH
CH
2
OH
OH
O
(CH
2
)
n
CH
3
O
Product Information
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Please note: Sucrose monoalkyl ester is a mixture of the three possible primary alcohol esters. Only one structure is shown here.
The Mega detergents are another series of glucose derivatives with an amide linkage between the alkyl tail and the
glycoside head group:
The Megas have been available for twenty years and have been used in numerous studies.
1,2,3,4
As sugar-based
detergents, they are rather easy to prepare and therefore reasonably priced. Unfortunately, their solubility is low and

decreases further as the alkyl chain is lengthened.
n = 6, MEGA-8
n = 7, MEGA-9
n = 8, MEGA-10
To overcome the low solubility, Anatrace has introduced a similar series:
HEGA
®
detergents
n = 6, HEGA
®
-8
n = 7, HEGA
®
-9
n = 8, HEGA
®
-10
n = 9, HEGA
®
-11
Note the presence of an additional hydroxyl group, which causes increased solubility.
O
CH
3
(CH
2
)
n
CH
3

C
CH
2
N
HO
CH
2
OH
C
C
C
C
H
H
H
H
OH
OH
OH
O
O
CH
2
OH
OH
OH
O
OH
OH
OH

CO
HOCH
2
CH
2
CH
3
(CH
2
)
n
O
n = 10, sucrose monododecanoate
n = 8, sucrose monodecanoate
O
C
CH
2
N
HO
CH
2
OH
C
C
C
C
H
H
H

H
OH
OH
OH
(CH
2
)
2
OH
CH
3
(CH
2
)
n
1. J. E. K. Hildreth, Biochem. J. 207, 363-366, (1982).
2. W. H. Yue, Y. P. Zou, L. Yu and C. A. Yu, Biochemistry 30, 2303-2306, (1991).
3. M. Hanatani, K. Nishifuji, M. Futai and T. Tsuchiya, J. Biochem. 95, 1349-1353, (1984).
4. D. M. Cortes and E. Perozo, Biochemistry 36, 10343-10352, (1997).
Product Information
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Detergents Designed for Membrane Proteins
CYMAL
®
Detergents
1
Anatrace has developed more than two dozen detergents designed specifically for the
extraction, purification and crystallization of membrane proteins. Support for the
effort described here came from NIH through the Small Business Innovative Research

(SBIR) program (NIDDK 1R43 DK43172). The first series made available was the
CYMAL
®
detergents which have the formula:
Length (Angstroms) Number of Carbon Atoms
! Cyclic Detergents ! Aliphatic Detergents
CMC (mM)
The CMC values for these detergents are higher than for the corresponding linear chains
containing the same number of carbons. Viewed another way, the cyclohexyl tails pack
more hydrophobicity into a shorter effective chain length. Unpublished data from Dr. J. E.
Harlan and Professor R. M. Garavito (Michigan State University) suggest that detergents
that have a shorter chain length, but higher hydrophobicity, are useful for stabilizing proteins
during crystallization.
A study published in late 1999 by the Dana Farber Cancer Institute and Harvard Medical
School showed that from the patented CYMAL
®
group of detergents, CYMAL-5
®
may
be the detergent of choice for separating and purifying G-protein coupled receptors.
2
1. US Patent 5,674,987 and US Patent 5,763,586.
2. Mirzabebov, T., Bannert, N., Farzan, M., Hofmann, W., Kolchisky, P., Wu, Lu.,
Ayalt, R. and Sodroski, I., J. of Biological Chemistry 274, 28745-28750 (1999).
O
O
O
(CH
2
)

n
O
CH
2
OH
OH
HO
OH
CH
2
OH
OH
OH
Product Information
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Polyoxyethylene Detergents
Most polyoxyethylene detergents used in the early studies of membrane proteins were
developed years ago for large industrial applications. Today an increasing number of well
defined, highly purified detergents, designed specifically for the extraction of membrane
proteins in their active state are available. Detergents such as dodecyl-β-D-
maltopyranoside and octyl-β-D-glucopyranoside have replaced many polyoxyethylene
detergents. Yet for some proteins the polyoxyethylene detergents remain very useful—
particularly for routine extraction procedures.
Polyoxyethylene detergents are available in a variety of structures and under an often
confusing list of trade names, such as TRITON
®
, TWEEN
®
, GENAPOL

®
, BRIJ
®
,
THESIT
®
, LUBROL
®
, etc. In addition to the confusion caused by trade names, industrial
detergents are often a mixture of closely related detergents that may vary from lot to lot.
They may also contain additives and contaminants which result in undesirable effects during
protein extraction. One such contaminant is an oxidizing agent.
1
These oxidants can
increase in concentration upon aging.
The “aging” of polyoxyethylene detergents results from the tendency of ethers to react with
oxygen to form peroxides. Light accelerates this process.
2-4
In samples of polyoxy-
ethylene detergents, hydrogen peroxide and organic peroxides with a variety of structures
may be found.
2-4
The concentration of hydrogen peroxide equivalent can be as high as
0.2%.
5
Conversely, it may be found that the level of peroxides has not increased over time
because as they form they react with more detergent molecules, resulting in the presence of
several undesirable derivatives.
2
The presence of peroxides during extraction of membrane proteins has resulted in inactiva-

tion, activation and even fragmentation of biological materials.
4
Sulfhydryl groups are
readily oxidized by peroxides and such oxidation induces aggregation of acetylcholine
receptors.
6
Adding to the complexity, sulfhydryl oxidation is implicated in the transition
from a conformation which has a high affinity for acetylcholine to a low affinity form. This
transition has been noted during the purification process.
7,8
Interference in biochemical analysis can also be caused by peroxides in detergent solu-
tions. Lever has reported that the Kober reagent, used to measure estrogen, reacted with
detergent solutions containing peroxides to give brown solutions even without heating.
2
Peroxides are probably responsible for the high blanks noted by Heath and Tappel when
measuring lipid peroxides in detergent-solubilized membrane components.
9
Even protein
determinations can be affected, as noted by Stutzenberger for COOMASSIE
®
blue dye-
binding assay, bicinchoninic acid method and the Folin phenol method.
10
1. Chang, H. W. and Bock, E., Anal. Biochem. 104, (1980), 112-117.
2. Lever, M., Anal. Biochem. 83, (1977), 274-284.
3. Miki, T. and Orii, Y., Anal. Biochem. 146, (1985), 28-34.
4. Jaeger, J., Sorensen, K. and Wolff, J., Biochem. Biophys. Methods 29, (1994), 77-81.
5. Ashani, Y. and Catravas, G., Anal. Biochem. 109, (1980), 55-62.
6. Chang, H. W., Proc. Nat. Acad. Sci. USA 71, (1974), 2113-2117.
7. O’Brien, R. D. and Gibson, R. E., ABB 169, (1975), 458-463.

8. Chang, H. W. and Neumann, E., Proc. Nat. Acad. Sci. USA 73, (1976), 3364-3368.
9. Heath, R. L. and Tappel, A. L., Anal. Biochem. 76, (1976), 184-191.
10. Stutzenberger, F. J., Anal. Biochem. 207, (1992), 249-254.
Product Information
INDEX PRODUCT LISTING NEW PRODUCTS PRODUCT INFOMATION
18 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
These detergents have been chromatographically purified to contain less than 20 µM of
equivalent peroxide in a 10% solution. Each detergent is supplied in a 10% aqueous
solution under argon gas in amber ampules.
50 ampules containing 1 ml of a 10% solution 61.41
5 ampules containing 10 ml of a 10% solution 51.17
10 ampules containing 10 ml of a 10% solution 92.10
500 ml of 10% solution in a screw cap bottle 369.32
Please note: ANAPOE
®
detergents are prepared from industrial detergents which
are often mixtures of closely related detergents. Even though ANAPOE
®
deter-
gents are purified by chromatography, they are not necessarily homogenous.
See ANAPOE
®
on pages 25-29 for pricing.
Cat. No. Description
APT020 ANAPOE
®
-20 (TWEEN
®
20)
APB035 ANAPOE

®
-35 (BRIJ
®
-35)
APB058 ANAPOE
®
-58 (BRIJ
®
-58)
APT080 ANAPOE
®
-80 (TWEEN
®
80)
APO106 ANAPOE
®
-C
10
E
6
(Polyoxyethylene(6)decyl ether)
APO109 ANAPOE
®
-C
10
E
9
(Polyoxyethylene(9)decyl ether)
APO128 ANAPOE
®

-C
12
E
8
(Polyoxyethylene(8)dodecyl ether)
Cat. No. Description
APO129 ANAPOE
®
-C
12
E
9
(Polyoxyethylene(9)dodecyl ether)
AP1210 ANAPOE
®
-C
12
E
10
(Polyoxyethylene(10)dodecyl ether)
APO138 ANAPOE
®
-C
13
E
8
(Polyoxyethylene(8)tridecyl ether)
APX100 ANAPOE
®
-X-100 (TRITON

®
X-100)
APX114 ANAPOE
®
-X-114 (TRITON
®
X-114)
APX305 ANAPOE
®
-X-305 (TRITON
®
X-305)
APX405 ANAPOE
®
-X-405 (TRITON
®
X-405)
These ANAPOE
®
detergents are also available in kits:
DSOL-ANAP1 ANAPOE
®
MASTER KIT (1 mL size) $29.00
Kit contains 1 mL of all 14 ANAPOE
®
detergents
DSOL-ANP10 ANAPOE
®
MASTER KIT (10 mL size) $79.00
Kit contains 10 mL of all 14 ANAPOE

®
detergents
DSOL-ANPC ANAPOE
®
CYEX KIT (10 mL size) $49.00
Kit contains 10 mL of ANAPOE
®
-35, ANAPOE
®
-58, ANAPOE
®
-C
10
E
6
,
ANAPOE
®
-C
10
E
9
, ANAPOE
®
-C
12
E
8
, ANAPOE
®

-C
12
E
9
,
ANAPOE
®
-C
12
E
10
, and ANAPOE
®
-C
13
E
8
.
DSOL-ANPT ANAPOE
®
TRITON/TWEEN KIT (10 mL size) $29.00
Kit contains 10 mL of ANAPOE
®
-X-100, ANAPOE
®
-X-114,
ANAPOE
®
-X-305, ANAPOE
®

-X-405, ANAPOE
®
-20, and ANAPOE
®
-80.
In each Kit detergents are supplied in a 10% aqueous solution under argon gas in amber ampules.
Product Information
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 19
Sherpas
®
- Polymeric Solubilization Aids
PMAL
™
-C12 was the first of a series of new polymeric reagents to be offered by Anatrace. PMAL
™
-C12 and the series that followed
were first prepared and characterized under an NIH-SBIR grant (GMR4460071) and are classified as amphipols. These polymers have
a unique way of solubilizing an integral membrane protein.
1-4
Amphipols wrap themselves around the hydrophobic transmembrane
domains of membrane proteins so that the hydrophobic side chains of the polymer stabilize the hydrophobic surface of the protein,
while the polar side chains of the polymer confer aqueous solubility to the protein-amphipol complex.
Recent papers describe the use of PMAL
™
-C12 for delivering complex integral membrane proteins (IMPs) into a membrane.
5,6
Applications can be imagined where it would be desirable to add a purified membrane protein to lipid vesicles or biological mem-
branes under conditions where the protein could spontaneously insert and adopt its functionally active state without lysing the
target bilayer.

These polymers appear to have some of the functions of the naturally occurring molecules called chaperones.
7,8
Since they are
synthetic polymers and not naturally occuring, the mechanism of transport may be different than chaperones. We are calling our
synthetic transport aids Sherpas
®
, after the mountain people who transport items in difficult environments.
See pages 89-91 in the Product Listing section for information on the series of PMAL
™
detergents.
An interesting new product is PMALA
™
-C12 which substitutes an Anzergent
®
(see pages 30-31) functionality for the simple
quatenary amine group in the PMAL
™
series. The structure of the first PMALA
™
offering is on page 91.
1. Tribet, C., Audebert, R. and Popot. J L., Proc. Nat. Acad. Sci 93, Amphipols: polymers that keep membrane proteins soluble in
aqueous solution, 15047-15050 (1996).
2. Tribet, C., Audebert and R., Popot. J L., Langmuir 13, Stabilization of hydrophobic colloidal dispersions in water with amphiphilic
polymers: application to integral membrane proteins, 5570-5576 (1997).
3. Tribert, C., Mills, D., Haiders, M. and Popot, J L., Biochemie 80, Scanning transmission electron microscopy study of the molecular
mass of amphipol/cytochrome b6f complexes, 475-482 (1998).
4. Champeil, P., Menguy, T., Tribet, C., Popot, J L. and le Marie, M., J. Biol. Chem. 275, Interaction of amphipols with sarcoplasmic
retitculum calcium Ca
2+
-ATPase, 18623-18637 (2000).

5. Nagy, J. K., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Oxenoid K. and Sanders, C. R., FEBS Letters 501, Use of amphipathic
polymers to deliver a membrane protein to lipid bilayers, 115-120 (2001).
6. Gorzelle, Bonnie M., Hoffmann, Amy Kuhn, Keyes, Melvin H., Gray, Don N., Ray, Dale G. and Sanders, Charles R.,
J. Am. Chem. Soc. 124, Amphipols Can Support the Activity of a Membrane Enzyme, 11594-11595 (2002).
7. Johnson, J. L. and Craig, E. A., Methods Enzymol. 351, Identifying functional interactions with molecular chaperones, 442-453 (2002).
8. Morello, J. P., Petaja-Repo, U. E., Bichet, D. G., and Bouvier, M., Trends Pharmacol. Sci. 21, Pharmacological chaperones: a new twist
on receptor folding, 466-469 (2000).
9. Popot, J. L., Berry, E. A., Charvolin, D., Creuzenet, C., Ebel, C., Engelman, D. M., Flotenmeyer, M., Giusti, F., Gohon, Y., Hervi, P.,
Hong, Q., Lakey, J. H., Leonard, K., Shuman, H. A., Timmins, P., Warschawski, D. E., Zito, F., Zoonens, M., Pucci, B., Tribet, C.,
Cell. Mol. Life Sci. 60, Amphipols: polymeric surfactants for membrane biology research, 1559-1574 (2003).
10. Cuccia, L., Trends Biochem. Sci. 27, Amphipols: polymers that can keep membrane proteins active in aqueous solution,
550-550 (2002).
11. Martinez, K. L., Gohon, Y., Corringer, P. J., Tribet, C., Merola, F., Changeux, J. P., and Popot, J. L., FEBS Lett. 528, Allosteric
transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical
constraints, 251-256 (2002).
12. Prata, C., Giusti, F., Gohon, Y., Pucci, B., Popot, J. L., and Tribet, C., Biopolymers 56, Nonionic amphiphilic polymers derived
from this (hydroxymethyl)acrylamidomethane keep membrane proteins soluble and native in the absence of detergent, 77-84 (2000).
CH
CH CH
2
C
CO
2
N
H
H
3
C
CH
3

CH
(CH
2
)
x
CH
3
)
n
(
(CH
2
)
3
NH
O
+
_
x=3, PMAL
TM
-C4
x=7, PMAL
TM
-C8
x=9, PMAL
TM
-C10
x=11, PMAL
TM
-C12

x=5, PMAL
TM
-C6
x=15, PMAL
TM
-C16
Product Information
INDEX PRODUCT LISTING NEW PRODUCTS PRODUCT INFOMATION
20 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
Biochemicals
MTS Reagents
The ability of MTS reagents (Methanethiosulfonate) to react very rapidly and specifically with cysteine groups
1-3
allows the quantitative derivatization of transmembrane proteins. The modification of the functional group attached to
the methylthiosulfonate results in a variety of sulfhydryl specific reagents that can probe the structure of a protein. For
example, charged MTS reagents are soluble in water and insoluble in a hydrophobic environment. Reaction of
charged MTS reagents indicates that the cysteine group is probably on or near the surface and not buried in the
interior of the protein or membrane. Other MTS reagents contain a spin labeled or a fluorescent group to probe the
protein/membrane environment.
4,5
Using site directed mutagenesis, one can introduce cysteine residues, one at a time, along a continuous stretch of
residues. Modification of residues, particularly with charged MTS reagents, gives information about the accessibility
of the residue in the ion channel. This technique was pioneered by Arthur Karlin and coworkers.
2,3,6,7
SCAM, or
substituted cysteine accessibility method, uses a small, polar, MTS reagent to modify each expressed mutant. The
accessibility of the particular residue is inferred from the irreversible functional effects of these reagents.
Anatrace is pleased to offer three charged MTS reagents:
A110MTS 2-AMINOETHYL METHANETHIOSULFONATE HYDROBROMIDE
(MTSEA)

FW 236.15 C
3
H
9
NO
2
S
2
HBr
S110MTS SODIUM (2-SULFONATOETHYL) METHANETHIOSULFONATE
(MTSES)
FW 236.18 C
3
H
7
NaO
5
S
3
T110MTS [2-(TRIMETHYLAMMONIUM)ETHYL] METHANE THIOSULFONATE
BROMIDE (MTSET)
FW 278.24 C
6
H
16
BrNO
2
S
2
For more information see the MTS reagents in the Alphabetical Product Listing section.

1. Kenyon, G. L. and Bruice, T. W. in Methods Enzymol. 47-Part E, 407-430 (1977).
2. Akabas, M. H., Stauffer, D. A., Xu, M. and Karlin, A., Science 258, 307-310 (1992).
3. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994).
4. Berliner, L. J., Grunwald, J., Hankovszky, H. O. and Hideg, K., Anal. Biochem. 119, 450-455 (1982).
5. Mannuzzu, L. M., Moronne, M. M. and Isacoff, E. Y., Science 271, 213-216 (1996).
6. Akabas, M. H., Kaufman, C., Archdeacon, P. and Karlin, A., Neuron 13, 919-927 (1994).
7. Zhang, H. and Karlin, A., Biochem. 36, 15856-15864 (1997).
These charged MTS reagents are also available in kits:
MT399-SMTS 51.30
(Each kit contains 100 mg of MTSEA, MTSES, and MTSET)
MT399-LMTS 192.38
(Each kit contains 500 mg of MTSEA, MTSES, and MTSET)
Product Information
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 21
NDSB Reagents
The series of NDSB's has been found to aid in the solubilization of some proteins. All members of the series, which
are zwitterionic over a pH range of 3-12 and are very water soluble
1
, have the unique ability to reduce protein aggre-
gation while stabilizing protein structure.
2
Other applications include refolding and renaturation of denatured proteins,
isoelectrofocusing, and capillary electrophoresis. In addition, NDSB compounds are of benefit in some protein
crystallizations.
3,4,5
1. Vuillard, L., Madern, D., Franzetti, B. and Rabilloud, T., Anal. Biochem. 230, 290-294 (1995).
2. Vuillard, L., Baalbaki, B., Lehmann, M., Norager, S., Legrand, P. and Roth, M., Journal of Crystal Growth 168, 150-154 (1996).
3. Vuillard, L., et al, Biochem. J. 305, 337 (1995).
4. Vuillard, L., et al, FEBS Letters 353, 294 (1994).

5. Righetti, P.G., et al, J. Capillary Electrophoresis 4, 47 (1997).
Anatrace is pleased to offer five NDSB reagents:
ND195 NDSB-195
(Dimethylethylammoniumpropanesulfonate)
FW 195.3 C
7
H
17
NO
3
S
ND201 NDSB-201
[(3-1-Pyridino)-1-propanesulfonate / 1-(3-Sulfopropyl)-pyridinium hydroxide, inner salt]
FW 201.2 C
8
H
11
NO
3
S
ND211 NDSB-211
[Dimethyl(2-hydroxyethyl)ammonium-1-propanesulfonate, 98%]
FW 211.3 C
7
H
17
NO
4
S
ND221 NDSB-221

[3-(1-Methylpiperidinium)-1-propanesulfonate]
FW 221.3 C
9
H
19
NO
3
S
ND256 NDSB-256
(Dimethylbenzylammoniumpropanesulfonate)
FW 257.4 C
12
H
19
NO
3
S
For more information see the NDSB reagents in the alphabetical Product Listing section.
These NDSB reagents are also available in kits:
ND399-1 (1g size) 39.10
(Each kit contains 1 g of NDSB-195, NDSB-201, NDSB-211, NDSB-221, and NDSB-256)
ND399-5 (5g size) 170.88
(Each kit contains 5 g of NDSB-195, NDSB-201, NDSB-211, NDSB-221, and NDSB-256)
Product Information
INDEX PRODUCT LISTING NEW PRODUCTS PRODUCT INFOMATION
22 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
Additional biochemicals offered by Anatrace are listed below:
B1010 BAKER'S DIMERCURIAL 32
B2000 X-GAL, ANAGRADE
®

103
B2010 MAGENTA-GAL 75
B2020 X-GLUC, CHA SALT 104
B2030 X-GLUC, Na SALT 104
B2040 BLUE-GAL 33
C3000 CMC DYE SOLUTION 38
C6500 CULTURE TUBES 38
CA100 CARBENICILLIN DISODIUM SALT 34
CH200 CHOLESTEROL 37
CY625 5-CYCLOHEXYL-1-PENTANOL 40
CY626 6-CYCLOHEXYL-1-HEXANOL 40
CY627 7-CYCLOHEXYL-1-HEPTANOL 40
D2000 DTT, CLELAND'S REAGENT 61
D3010 1,2-DIMYRISTOYL-RAC-GLYCEROL, ANAGRADE
®
54
D3012 1,2-DILAUROYL-RAC-GLYCEROL, ANAGRADE
®
52
D3014 1,2-DIDECANOYL-RAC-GLYCEROL, ANAGRADE
®
52
M2500 MICROPIPETTES 77
S1001 SODIUM DODECYL SULFATE, BIOTECH GRADE 94
S2000 L-(+)-SELENOMETHIONINE, ANALYTICAL GRADE
®
92
T1010 TRIMETHYL LEAD ACETATE 99
T1020 TRIETHYL LEAD ACETATE 99
T2033 TAUROCHOLIC ACID, ANAGRADE

®
95
Books
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 23
BOOKS
Anatrace is pleased to offer the following texts to our researchers. You
may order your books separately or include them with your regular order.
BOOK001 Methods and Results in Crystallization of Membrane Proteins 109.95
Edited by So Iwata
Part I: Introduction
Part II: Principles and Techniques in Membrane Protein Crystallization
Part III: Examples of Successful Crystallization of Membrane Proteins
Part IV: Crystallization Informatics of Membrane Proteins
"Membrane protein crystallisation is still one of the hardest challenges
in Structural Biology. This book is written to assist the researchers
attempting to crystallize their own membrane proteins."
- from book Abstract (Introduction)
BOOK002 Membrane Protein Protocols: 99.50
Expression, Purification, and Characterization
Edited by Barry S. Selinsky
A collection of key techniques for the study of receptors and transport proteins.
The book provides examples of how different membrane proteins can be over-
expressed in both prokaryotic and eukaryotic expression systems, how natural
and overexpressed proteins can be solubilized from their host membranes, and
how the solubilized protein can be purified in active form. Each protocol contains
step-by-step instructions to ensure success, troubleshooting advice, lists of reagents,
and tips on avoiding pitfalls.
BOOK003 Protein Structure and Function 49.95
Petsko and Ringe

This text introduces general principles of protein structure, folding, and function,
then goes beyond these basics to tackle the conceptual basis of inferring structure
and function from genomic sequence. Written for upper-level undergraduates and
beginning graduate students, Protein Structure and Function will also be useful
for working scientists needing an up-to-date introduction to the field.
(Available in the U.S. and Canada only)
BKPKG1 All Three Books (10% Discount) 233.46
(Available in the U.S. and Canada only)
New Products
INDEX PRODUCT LISTING NEW PRODUCTS PRODUCT INFOMATION
24 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com
NEW PRODUCTS
The following new Detergents are listed in this catalog:
Detergent Catalog # Page #
n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE
®
D365 47
n-Decyl-α-D-maltopyranoside, ANAGRADE
®
(High Alpha) D322HA 49
n-Decyl-β-D-maltopyranoside, ANAGRADE
®
(Low Alpha) D322LA 49
Decyltrimethylammonium chloride D354 51
n-Dodecyl-α-D-glucopyranoside, ANAGRADE
®
(High Alpha) D318HA 56
n-Dodecyl-β-iminodipropionic acid, disodium salt D345 57
n-Dodecyl-β-iminodipropionic acid, monosodium salt D346 57
Dodecyltrimethylammonium chloride D355 60

FOS-CHOLINE
®
-14, DEUTERATED F312D 65
Hexadecyltrimethylammonium chloride H355 73
Octadecyltrimethylammonium chloride O355 81
n-Octyl-α-D-glucopyranoside, ANAGRADE
®
(High Alpha) O311HA 83
Tetradecyltrimethylammonium chloride T355 97
n-Undecyl-α-D-maltopyranoside, ANAGRADE
®
(High Alpha) U300HA 101
The following new Reagents are listed in this catalog:
Reagent Catalog # Page #
DTT, Cleland's Reagent D2000 61
NDSB-211 ND211 78
NDSB-221 ND221 78
The following new Kits are listed in this catalog:
Kit Catalog # Page #
High Alpha Detergent (1 g size) D399-HA 7
Quaternary Detergent Kit (1 g size) D399-QUAT 8
NDSB Reagent Kit (1 g size) ND399-1 21
NDSB Reagent Kit (5 g size) ND399-5 21
High Alpha Detergent (Solution) DSOL-HA 10
Anapoe
®
Master Kit (1 mL size) DSOL-ANAP1 10
Anapoe
®
Master Kit (10 mL size) DSOL-ANP10 10

Anapoe
®
CYEX Kit DSOL-ANPC 10
Anapoe
®
Triton/Tween Kit DSOL-ANPT 10
Quaternary Detergent Kit (Solution) DSOL-QUAT 11
800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 25
Product Listing
Catalog No. Product Name/Specifications Size Price
SERVICE INFORMATION PRODUCT INFORMATION NEW PRODUCTS INDEX
A110MTS 2-AMINOETHYL METHANETHIOSULFONATE HYDROBROMIDE 100 mg 28.50
[MTSEA] 500 mg 106.88
1 g 192.38
FW 236.15 C
3
H
9
NO
2
S
2
•
HBr
Solubility in water at 0°-5°C ≥ 10%
.
HBr
CH
3
S

S
O
O
(CH
2
)
2
NH
2
Positively charged reagent that reacts very rapidly
and specifically with cysteine groups.
Approximate molecular dimensions: This reagent would fit inside
a cylinder about 0.6 nm in diameter and 1 nm in length.
1
Half-life (pH 7.0, 20°C) ~ 12.0 minutes
2
Half-life (pH 6.0, 20°C) ~ 92.0 minutes
2
Half-life (pH 7.0, 4°C) ~ 116.0 minutes
2
1. Akabas, M. H., Stauffer, D. A., Xu, M. and Karlin, A., Science 258, 307-310 (1992).
2. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998).
3. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003).
A340 ANAMEG
®
-7, ANAGRADE
®
1 g 35.51
[Methyl-6-O-(N-heptylcarbamoyl)-α-D-glucopyranoside / HECAMEG / 5 g 142.04
6-O-(N-Heptylcarbamoyl)-methyl-α-D-glucopyranoside] 25 g 568.16

FW 335.4 [115457-83-5] C
15
H
29
NO
7
CMC (H
2
O) ~ 19.5 mM
1
(0.65%)
Greater than 98% pure by HPLC analysis.
O

OCH
3

C
O
H
(CH
2
)
6
CH
3
N
HO
CH
2

O
OH
OH
pH (1% solution) 5-9
Solubility in water at 0-5°C ≥ 10%
Conductance (10% solution) < 80 µS
Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
Absorbance of a 1% detergent solution: 340 nm < 0.03
280 nm < 0.05
260 nm < 0.07
225 nm < 0.1
1. Plusquellec, D., Chevalier, G., Talibart, R. and Wroblewski, H., Anal. Biochem. 179, 145-153 (1989).
APT020 ANAPOE
®
-20 50 ml (50 ampules/1 ml each) 61.41
[TWEEN
®
20 / Polyoxyethylene(20)sorbitan monolaurate / 50 ml (5 ampules/10 ml each) 51.17
Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate] 100 ml (10 ampules/10 ml each) 92.10
500 ml (screw cap bottle) 369.32
FW avg. 1228 [9005-64-5]
CMC ~ 0.059 mM
1
(0.0072%)
O
(OCH
2
CH
2
)

x
OH
HO(CH
2
CH
2
O)
w
CH(OCH
2
CH
2
)
y
OH
CH
2
O(CH
2
CH
2
O)
z
Sum of w + x + y + z = 20
R
R = C
11
H
23
CO

2
(TWEEN 20)
OXIDANT-FREE
Purified industrial detergent—may contain several components.
Chromatographically purified to contain 20 µM of equivalent peroxide.
Supplied in a 10% (w/v) solution under argon gas.
1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979).