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BÁO CÁO-DNA vaccine and Application of DNA vaccine in Fisheries

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DNA vaccine and Application of
DNA vaccine in “Fisheries”
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by Activated User
Photo Album
by Activated User

DNA vaccine is DNA sequence used
as a vaccine

This DNA sequence code for
antigenic protein of pathogen

As this DNA inserted into cells it is
translated to form antigenic protein.
As this protein is foreign to cells, so
immune response raised against this
protein.

In this way, DNA vaccine provide
immunity against that pathogen.
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by Activated User
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IHN is an RNA stranded virus. The genome codes for 6 different proteins are given.
Five are structural proteins and one is a non structural protein.
Must know which gene codes for the protein that is involved in the immunetic
response. It is the glyco protein spike on the outside of the virus, denoted by the G
protein, that is the immunetic protein of the virus.
Insect G-gene into DNA plasmid form Recombinant DNA
Inject DNA vaccine into Fish
G-gene construct shows good surface expression, which is believed to be a key
feature to the effectiveness of the vaccine. You also see a very heavy monocytic
cell infiltrate, most likely white blood cells.

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